puromycin selectable marker (TaKaRa)
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TaKaRa
puromycin selectable marker
Puromycin Selectable Marker, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puromycin+selectable+marker/Puromycin/bio_rxiv__2025__06__13__659598-167-25-36
Average 96 stars, based on 3509 article reviews
Puromycin Selectable Marker, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/puromycin+selectable+marker/Puromycin/bio_rxiv__2025__06__13__659598-167-25-36
Average 96 stars, based on 3509 article reviews
puromycin selectable marker - by Bioz Stars,
2026-10
96/100 stars
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Expressing:Article Title: Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses Article Snippet: Sequences for the sgRNAs ( Supplementary Table 1 ) were designed using tools provided by the Zhang Lab at the Broad Institute (available at http://crispr.mit.edu/ ). .. B16-GFP-Cas9 cells were co-transfected with 2 sgRNAs expression vectors targeting Trp2 and a plasmid expressing EGFP and a Plasmid Preparation:Article Title: Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses Article Snippet: Sequences for the sgRNAs ( Supplementary Table 1 ) were designed using tools provided by the Zhang Lab at the Broad Institute (available at http://crispr.mit.edu/ ). .. B16-GFP-Cas9 cells were co-transfected with 2 sgRNAs expression vectors targeting Trp2 and a plasmid expressing EGFP and a Article Title: The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes Article Snippet: All constructs were confirmed by DNA sequencing. pGEX 6P-4 vector (GE Healthcare Life Science) or pDuet1 His-HRV3C and pDuet1 His-HRV3C-AviTag were used to express GST- or His-tagged proteins, respectively, in bacteria. pEF1a-GST-6P-4 or pEF1a-GST-P-N4 vector was used to express amino terminal or carboxyl terminal GST-tagged proteins in 293T cells. pFLAG-C4 was derived from pMYC-C4 ( ) by replacing the MYC tag with FLAG tag. .. This plasmid was used to transfect 293T for the overexpression of protein. pMSCV-neo MYC-C4 retroviral vector was derived from pMSCV-puro myc vector by replacing the Article Title: Functional Coupling between Secretory and Cytosolic Phospholipase A2 Modulates Tumor Necrosis Factor-α- and Interleukin-1β-induced NF-κB Activation Article Snippet: .. HaCaT cells were transfected using the calcium phosphate precipitation procedure according to standard protocols using a 10-fold excess of the plasmid of interest (pBIIX or pfLUC) over the selection plasmid encoding a Marker:Article Title: Eradication of large established tumors in mice by combination immunotherapy that engages innate and adaptive immune responses Article Snippet: Sequences for the sgRNAs ( Supplementary Table 1 ) were designed using tools provided by the Zhang Lab at the Broad Institute (available at http://crispr.mit.edu/ ). .. B16-GFP-Cas9 cells were co-transfected with 2 sgRNAs expression vectors targeting Trp2 and a plasmid expressing EGFP and a Article Title: The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes Article Snippet: All constructs were confirmed by DNA sequencing. pGEX 6P-4 vector (GE Healthcare Life Science) or pDuet1 His-HRV3C and pDuet1 His-HRV3C-AviTag were used to express GST- or His-tagged proteins, respectively, in bacteria. pEF1a-GST-6P-4 or pEF1a-GST-P-N4 vector was used to express amino terminal or carboxyl terminal GST-tagged proteins in 293T cells. pFLAG-C4 was derived from pMYC-C4 ( ) by replacing the MYC tag with FLAG tag. .. This plasmid was used to transfect 293T for the overexpression of protein. pMSCV-neo MYC-C4 retroviral vector was derived from pMSCV-puro myc vector by replacing the Article Title: Functional Coupling between Secretory and Cytosolic Phospholipase A2 Modulates Tumor Necrosis Factor-α- and Interleukin-1β-induced NF-κB Activation Article Snippet: .. HaCaT cells were transfected using the calcium phosphate precipitation procedure according to standard protocols using a 10-fold excess of the plasmid of interest (pBIIX or pfLUC) over the selection plasmid encoding a Over Expression:Article Title: The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes Article Snippet: All constructs were confirmed by DNA sequencing. pGEX 6P-4 vector (GE Healthcare Life Science) or pDuet1 His-HRV3C and pDuet1 His-HRV3C-AviTag were used to express GST- or His-tagged proteins, respectively, in bacteria. pEF1a-GST-6P-4 or pEF1a-GST-P-N4 vector was used to express amino terminal or carboxyl terminal GST-tagged proteins in 293T cells. pFLAG-C4 was derived from pMYC-C4 ( ) by replacing the MYC tag with FLAG tag. .. This plasmid was used to transfect 293T for the overexpression of protein. pMSCV-neo MYC-C4 retroviral vector was derived from pMSCV-puro myc vector by replacing the Retroviral:Article Title: The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes Article Snippet: All constructs were confirmed by DNA sequencing. pGEX 6P-4 vector (GE Healthcare Life Science) or pDuet1 His-HRV3C and pDuet1 His-HRV3C-AviTag were used to express GST- or His-tagged proteins, respectively, in bacteria. pEF1a-GST-6P-4 or pEF1a-GST-P-N4 vector was used to express amino terminal or carboxyl terminal GST-tagged proteins in 293T cells. pFLAG-C4 was derived from pMYC-C4 ( ) by replacing the MYC tag with FLAG tag. .. This plasmid was used to transfect 293T for the overexpression of protein. pMSCV-neo MYC-C4 retroviral vector was derived from pMSCV-puro myc vector by replacing the Derivative Assay:Article Title: The nucleus activates mechano-responsiveness via FHOD-associated LINC complexes Article Snippet: All constructs were confirmed by DNA sequencing. pGEX 6P-4 vector (GE Healthcare Life Science) or pDuet1 His-HRV3C and pDuet1 His-HRV3C-AviTag were used to express GST- or His-tagged proteins, respectively, in bacteria. pEF1a-GST-6P-4 or pEF1a-GST-P-N4 vector was used to express amino terminal or carboxyl terminal GST-tagged proteins in 293T cells. pFLAG-C4 was derived from pMYC-C4 ( ) by replacing the MYC tag with FLAG tag. .. This plasmid was used to transfect 293T for the overexpression of protein. pMSCV-neo MYC-C4 retroviral vector was derived from pMSCV-puro myc vector by replacing the Transfection:Article Title: Functional Coupling between Secretory and Cytosolic Phospholipase A2 Modulates Tumor Necrosis Factor-α- and Interleukin-1β-induced NF-κB Activation Article Snippet: .. HaCaT cells were transfected using the calcium phosphate precipitation procedure according to standard protocols using a 10-fold excess of the plasmid of interest (pBIIX or pfLUC) over the selection plasmid encoding a Selection:Article Title: Functional Coupling between Secretory and Cytosolic Phospholipase A2 Modulates Tumor Necrosis Factor-α- and Interleukin-1β-induced NF-κB Activation Article Snippet: .. HaCaT cells were transfected using the calcium phosphate precipitation procedure according to standard protocols using a 10-fold excess of the plasmid of interest (pBIIX or pfLUC) over the selection plasmid encoding a |